The platform of NMR of I2BC specialises in the characterisation of proteins in solution, whether folded or disordered. It has focused in particular on proteins involved in cell signalling, DNA damage repair and nuclear organisation: folding, dynamics, protein:protein and protein:ligand interactions, described at the atomic scale that this spectroscopy allows. The platform has also developed and exploited a new approach: the identification and residue-by-residue kinetic monitoring of post-translational modifications (PTMs). This approach allows the identification and characterisation of multiple neighbouring or degenerate modifications, and can be applied to virtually any type of PTM (more information: see Ehlen et al. Nat. Comm. 2020 / Alik et al, Angew. Chem. 2020). Finally, the platform uses NMR to map interfaces for the design of protein-protein interaction inhibitors (more information: see Bakail et al. Cell. Chem. Biol. 2019).
In terms of equipment, the platform has two high-field spectrometers (600 and 700 MHz) equipped with a 1H, 15N, 13C TCI cryo-probe, and is attached to FRISBI, the national infrastructure for integrative structural biology.
Finally, this platform is part of the I2BC's structural biology platform cluster, which includes: i) the Crystallization, NMR, CryoEM, and Macromolecular Interaction Measurements platforms, and ii) the technical platforms for Soluble or Membrane Protein Expression in Yeast, Protein Expression in Insect Cells, and Structural Bioinformatics.
Lab of attachment
CEA/DRF/JOLIOT, CEA Paris-Saclay, I2BC@SACLAY, SB2SM and I2BC (UMR 9198)
Offres de formations
Establishments of affiliation