Paris-Saclay screening (CAPS)

Technological platform

Description

Since 2018, the C@PS platform has been providing the scientific community (academic or industrial laboratories) with its skills and high-performance equipment for biological screening.
IBiSA-certified, the platform is committed to a continuous improvement approach with the aim of customer satisfaction and high-quality services.
The C@PS platform offers laboratories individualised support as part of collaborations or services. C@PS is based on 4 services (CIBI, QPCR-CTPF, CIBLOT, PC2B) offering complementary technologies to answer a wide range of scientific questions such as the study of protein-ligand interactions, transcriptome analysis, cytotoxicity measurements on healthy or tumour cells, in ovo screening, the synthesis of targeted chemical libraries and the identification of chemical modulators.

Contacts

  • Mr Jean-Christophe CINTRAT
  • Mr Eric JACQUET
  • Mr Jerome BIGNON

https://www.ibisa.net/plateformes/plateforme-criblage-paris-saclay-c-ps-582.html

Innovation themes

Map and access

C@PS CEA/DRF/JOLIOT/DMTS/SCBM, Bâtiment 547
91191 Gif-sur-Yvette

Technology and equipments

Technologies utilisées

High-throughput screening

In ovo screening

Chemical library screening

Cell culture

Bacterial testing

Biochemical tests

Quantitative PCR

Thermal shift assay

Chemical synthesis and medicinal chemistry: radioactive screening, hit optimisation, probe synthesis (target fishing),

- Characterisation of target proteins by thermal shift assay (TSA): screening of buffer, pH, salt and divalent conditions. Measurement of protein-ligand and protein-protein interactions.

- Study of the transcriptome by quantitative PCR: sample preparation and quality control, design of primers or hydrolysis probes, low- and high-throughput quantitative PCR, data processing and analysis, Cell biology (cell lines and primary cells): culture, cell viability and proliferation, cell death, cell cycle - Study of the effect of bioactive molecules in ovo - Bacterial tests: viability and photodynamic therapy.

Keywords

Formations offers

Types de prestations

  • Training

Offres de prestations

Cell tests: viability, apoptosis, necrosis, autophagy, cell cycle; High-throughput screening in 96 or 384 wells: protein-protein interactions; enzymatic tests; chemical library; in OVO screening: angiogenesis, tumour proliferation; bacterial tests; high-throughput quantitative PCR : target gene expression screening, pathologies and effect of therapeutic molecules, diagnostic markers, 96/384/TAC/OA format; thermal shift assay: thermostability, buffer optimisation, effect of mutations, protein-ligand interaction screening, identification of chemical modulators, etc.

Offres de formations

CNRS quantitative PCR training for companies, hosting students, PhD students and post-docs at the platforms to help them carry out their projects and training them to use the equipment.

Ecosystem

Establishments of affiliation

Labellisations

  • IBISA

Réseaux

  • GENOPS (Réseau des plateformes de GENOmique Paris-Saclay)